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KMID : 0613820100200111738
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Journal of Life Science
2010 Volume.20 No. 11 p.1738 ~ p.1741
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A New Bioluminescent Rat Prostate Cancer Cell Line: Rapid and Accurate Monitoring of Tumor Growth
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Lee Mi-Sook
Jung Jae-In
Kwon Seung-Hae
Shim In-Sop
Hahm Dae-Hyun
Han Jeong-Jun
Han Dae-Seok
Yoon Jung-Han
Her Song-Wook
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Abstract
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Caliper measurements of tumor volume have been widely used in the assessment of tumors in animal models. However, experiments based on caliper data have resulted in unreliable estimates of tumor growth, due to necrotic areas of tumor mass. To overcome this systematic bias, we engineered a new luciferase-expressing rat prostate cancer cell line (MLL-Luc) that produces bioluminescence from viable cancer cells. MLL-Luc cells showed a strong correlation between bioluminescence intensity and cell number (R²=0.99) and also accurately quantified tumor growth, with reduced bioluminescence signals caused by necrotic cells in a subcutaneous MLL-Luc xenograft model. The accurate quantification of tumor growth with bioluminescence imaging (BLI) was confirmed by a better antitumor effect of combination chemotherapy, compared to that based on caliper measurements with a correlation between the bioluminescence signal and tumor volume (R²=0.84). These data suggest that bioluminescent MLL xenografts are a powerful and quantitative tool for monitoring tumor growth and are useful in evaluating the efficacy of anticancer drugs, with less systematic bias.
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KEYWORD
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Bioluminescent imaging, luciferase, prostate cancer xenograft, rat MAT LyLu (MLL) cells
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